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Antibacterial and DPPH free radical-scavenging activities of methanolic extracts of Aaptos sp. (Marine Sponges)


Mohamad, Habsah and Mat Rashid, Zalilawati and Shaari, Khozirah and Latip, Jalifah and Lajis, Md Nordin and Ali, Abdul Manaf (2009) Antibacterial and DPPH free radical-scavenging activities of methanolic extracts of Aaptos sp. (Marine Sponges). Pertanika Journal of Tropical Agricultural Science, 32 (1). pp. 43-50. ISSN 1511-3701; ESSN: 2231-8542


This study reports on the evaluation of the antioxidant and antibacterial activities of twelve methanolic extracts (A-L) of Aaptos sp., collected from various locations of Terengganu Islands, namely Pulau Bidong, Pulau Kapas, Pulau Perhentian and Pulau Redang. The antioxidant activity of the twelve specimens was evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method. The antibacterial bioassay against 5 bacteria, i.e. Bacillus subtilis (gram-positive), Escherichia coli (gram-negative), Bacillus proteus, Streptococus agalatea and Streptococus fecalis, was carried out using the disc-diffusion method. In the DPPH method, all extracts exhibited moderate and strong radical scavenging activity when compared to the standards used, i.e. quercetin and butylated hydroxyanisole (BHA) with the inhibition percentage in the range of 55–89%. In particular, specimen H exhibited the strongest radical scavenging activity with IC50 value of 0.1mg/ml. On the contrary, all the specimens showed antibacterial activity at least against one test organism. Interestingly, specimens C, G and L, which were collected from Perhentian, Bidong and Kapas Islands respectively, exhibited weak to strong activity against all bacterial strains. Beside that, specimen F (collected off Redang Island) was weakly bactericidal only against Bacillus proteus. Meanwhile, specimen G (collected off Bidong island) was primarily selected for further isolation to yield cholestan-3β-ol and aaptamine.

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Additional Metadata

Item Type: Article
Divisions: Institute of Bioscience
Publisher: Universiti Putra Malaysia Press
Keywords: DPPH free radical scavenging; Antibacterial; Aaptos sp.; Cholestan-3β-ol; Aaptamine
Depositing User: Muizzudin Kaspol
Date Deposited: 24 Jul 2017 10:16
Last Modified: 24 Jul 2017 10:16
URI: http://psasir.upm.edu.my/id/eprint/17996
Statistic Details: View Download Statistic

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