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Viability of bifidobacterium pseudocatenulatum G4 after spray-drying and freeze-drying


Abd Manap, Mohd Yazid and Shobirin, Anis and Mohamad, Rosfarizan and Mustafa, Shuhaimi and Wong, Stephenie and Kabeir, Barka Mohammed (2009) Viability of bifidobacterium pseudocatenulatum G4 after spray-drying and freeze-drying. Microbiology Insights, 2 (July). pp. 1-7. ISSN 1178-6361

Abstract / Synopsis

Viability of Bifidobacterium pseudocatenulatum G4 following spray-drying and freeze-drying in skim milk was evaluated. After spray-drying, the strain experienced over 99% loss in viability regardless of the air outlet temperature (75 and 85°C) and the heat-adaptation temperature (45 and 65 °C, 30 min). The use of heat-adaptation treatment to improve the thermotolerance of this strain was ineffective. On the other hand, the strain showed a superior survival at 71.65%–82.07% after freeze-drying. Viable populations of 9.319–9.487 log10 cfu/g were obtained when different combinations of skim milk and sugar were used as cryoprotectant. However, the addition of sugars did not result in increased survival during the freeze-drying process. Hence, 10% (w/v) skim milk alone is recommended as a suitable protectant and drying medium for this strain. The residual moisture content obtained was 4.41% ± 0.44%.

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Additional Metadata

Item Type: Article
Subject: Skim milk
Subject: Dairy products
Divisions: Faculty of Food Science and Technology
DOI Number:
Publisher: Libertas Academica Ltd.
Keywords: Bifidobacteria; Freeze-drying; Cryoprotectant; Spray-drying; Survival; Viability
Depositing User: Khairil Ridzuan Khahirullah
Date Deposited: 05 Dec 2011 11:07
Last Modified: 30 Nov 2015 15:31
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