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Validated high performance liquid chromatographic (HPLC) method for analysis of zerumbone in plasma


Mohammad Eid, Eltayeb Elamin and Abdul, Ahmad Bustamam and Al-Zubairi, Adel Sharaf and Sukari, Mohamed Aspollah and Abdullah, Rasedee (2010) Validated high performance liquid chromatographic (HPLC) method for analysis of zerumbone in plasma. African Journal of Biotechnology, 9 (8). art. no. 001C7D637307. pp. 1260-1265. ISSN 1684–5315


Zerumbone (ZER) is a sesquiterpene derived from Zingiber zerumbet smith, family Zingiberaceae. It has been shown to possess anti-cancer and apoptosis-inducing properties against various human tumour cells as well as in vivo against a number of induced malignancies in mice. In this study a simple, specific and accurate high performance liquid chromatographic method for determination of ZER in micro-volumes human plasma (| 1.5 ml) was developed and validated. ZER and its analogue α-Humulene as internal standard were easy recovered by simple one step plasma protein precipitation using acetonitrile and separated in isocratic mobile phase, on reverse phase-C18 column. The effluent was monitored by Photodiode Array (PDA) detector and at a flow rate of 1.0 ml/min. The linearity of proposed method was 2 – 15 µg/ml. The intra-day and inter-day coefficient of variation and percent error values of the method were less than 15% and mean recovery was more than 90% for both ZER and α-Humulene. This method was found to be precise, specific, accurate and robust for detection and analysis of ZER in human plasma.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Science
Faculty of Veterinary Medicine
Institute of Bioscience
Publisher: Academic Journals
Keywords: ZER; Humulene; HPLC; Human plasma
Depositing User: Najwani Amir Sariffudin
Date Deposited: 15 Dec 2011 04:33
Last Modified: 15 Nov 2017 01:46
URI: http://psasir.upm.edu.my/id/eprint/17571
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