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Toxicity evaluation of dextran-spermine polycation as a tool for genetherapy in vitro.


Abedini, Fatemah and Ismail, Maznah and Hosseinkhani, Hossein and Tengku Ibrahim, Tengku Azmi and Omar, Abdolrahman and Chong, Pei Pei and Ismail, Norsharina and Farber, Ira-Yudovin and Domb, Abraham J. (2010) Toxicity evaluation of dextran-spermine polycation as a tool for genetherapy in vitro. Journal of Cell and Animal Biology, 4 (12). pp. 170-176. ISSN 1996-0867


Cationic polymers are a leading class of nonviral self-assembled nucleic acid delivery systems. Cationic polymers have been shown to condence the DNA so that the entrapped DNA is protected from contact with DNase. The objective of the present study is to evaluate the effect of cationic dextran on the proliferation rate, morphological changes and biosynthetic activities in vitro. Cationic dextran was prepared by means of reductive-amination between oxidized dextran and the natural oligoamine, spermine. Four kinds of biological evaluations including cell proliferation assay, ultrastructural changes of cells using transmission electron microscopy (TEM), acridine orange/Propidium Iodide and cell cycle were studied. Our results clearly indicated that the toxicity of cationic dextran is dose depended and it is not toxic at low concentration and tolerable by the cells, and it can be used as a tool for gene delivery.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Publisher: Academic Journals
Keywords: Dextran-spermine; Genetheraphy; Nonviral vectors; Toxicity.
Depositing User: kmportal
Date Deposited: 19 Nov 2013 02:54
Last Modified: 05 Nov 2015 08:07
URI: http://psasir.upm.edu.my/id/eprint/17451
Statistic Details: View Download Statistic

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