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The effect of plant growth regulators on optimization of tissue culture system in Malaysian upland rice


Shahsavari, Esmaeil and Abdul Aziz, Maheran and Abdullah, Siti Nor Akmar and Musa, Mohamed Hanafi (2010) The effect of plant growth regulators on optimization of tissue culture system in Malaysian upland rice. African Journal of Biotechnology, 9 (14). art. no. F05F94D20301. pp. 2089-2094. ISSN 1684–5315


Mature seeds of four upland rice cultivars namely Kusan, Lamsan, Selasi and Siam were assessed for callus induction and plant regeneration on different concentrations and combinations of plant growth regulators, incorporated into MS (Murashige and Skoog) basal medium. Callus induction frequency was significantly different among the cultivars, as well as among the 2,4-Dichlorophenoxyacetic acid (2,4-D) levels tested. All tested cultivars exhibited highest callus frequency at 2 mgl-1 2,4-D. The incorporation of α-naphthaleneacetic acid (NAA) and kinetin (Kin) in the callus induction medium supplemented with 2 mgl-1 2,4-D did not significantly improve the callus induction frequency. After two subcultures, at 24 days interval, the best response to callus induction was from cultivar Selasi, while callus browning became prominent in cultivars Kusan and Siam. Embryogenic callus placed on different regeneration media exhibited the highest regeneration frequency on medium containing 0.5 mgl-1 NAA + 2.0 mgl-1 Kin + 2.0 mgl-1 6-benzylaminopurine (BAP). The maximum regeneration frequency was achieved in cultivar Selasi followed by Lamsan while Siam and Kusan exhibited poor regeneration response. Among the four upland rice cultivars evaluated, Selasi and Lamsan are two promising cultivars in terms of callus induction frequency and morphology, and regeneration ability of the embryogenic callus.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Publisher: Academic Journals
Keywords: Callus induction frequency; Regeneration frequency; Plant growth regulators; Upland rice
Depositing User: Norhazura Hamzah
Date Deposited: 21 Jun 2012 07:32
Last Modified: 09 Nov 2017 09:16
URI: http://psasir.upm.edu.my/id/eprint/17099
Statistic Details: View Download Statistic

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