UPM Institutional Repository

The direct recovery of recombinant hepatitis B core antigen from disruptate derived from continuous-flow bead milling


Citation

Ho, Chin Woi and Tan, Wen Siang and Kamarudin, Suryani and Ling, Tau Chuan and Tey, Beng Ti (2008) The direct recovery of recombinant hepatitis B core antigen from disruptate derived from continuous-flow bead milling. Biotechnology and Applied Biochemistry, 50 (1). pp. 49-59. ISSN 0885-4513; ESSN: 1470-8744

Abstract

HBcAg (hepatitis B core antigen) is a nanoplex bioproduct that has a great potential in the development of therapeutic drugs and vaccines. In the present study, a continuous-flow bead milling for the disruption of Escherichia coli was optimized and a direct recovery protocol to isolate the recombinant HBcAg from the unclarified E. coli disruptate was developed. The optimal condition for continuous-flow bead milling for the release of HBcAg from E. coli was achieved at a feed flow rate of 15 litres/h, biomass concentration of 10% [ww/v (wet weight/vol.)] and impeller tip speed of 14 m/s. The sucrose-density-gradient analysis showed that the particulate form of the HBcAg released by this optimal condition is still preserved. In the direct purification of HBcAg from the unclarified disruptate, the AE-EBAC (anion-exchange expanded-bed adsorption chromatography) technique was employed. A 54% adsorption and 50.7% recovery of HBcAg were achieved in this direct recovery process. The purity of HBcAg recovered was 49.8%, which corresponds to a purification factor of 2.0. ELISA showed that the HBcAg recovered is functionally active.


Download File

[img]
Preview
PDF (Abstract)
The direct recovery of recombinant hepatitis B core antigen from disruptate derived from continuous.pdf

Download (84kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Engineering
Institute of Bioscience
DOI Number: https://doi.org/10.1042/BA20070088
Publisher: Portland Press
Keywords: Anion-exchange expanded-bed adsorption chromatography;(AE-EBAC); Continuous-flow bead milling; Escherichia coli; Hepatitis B core antigen (HBcAg); Hepatitis B virus (HBV); Therapeutic vaccine
Depositing User: kmportal
Date Deposited: 02 Jan 2014 05:35
Last Modified: 30 Nov 2016 09:36
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1042/BA20070088
URI: http://psasir.upm.edu.my/id/eprint/17060
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item