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Synthesis, characterization, and structural properties of intracellular copolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB 172 from renewable resource


Citation

Mumtaz, Tabassum and Abd. Aziz, Suraini and Phang, Lai Yee and Wan Yunus, Wan Md. Zin and Shirai, Yoshihito and Hassan, Mohd Ali (2010) Synthesis, characterization, and structural properties of intracellular copolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB 172 from renewable resource. International Journal of Polymer Analysis and Characterization, 15 (6). pp. 329-340. ISSN 1023-666X

Abstract

Microbial copolymer was produced by a local isolate, Comamonas sp. EB 172, using mixed organic acids such as acetic, propionic, and butyric acids as carbon sources in pH-stat fed-batch fermentation. Maximum polymer production (6.59 g/L) was achieved at 50 h of fermentation when 73.64 g/L mixed acids, generated from the acidogenic fermentation of palm oil mill wastewater, were used. Accumulation of polymer in the cell was 70% (wt/wt), which was observed under transmission electron microscope. The morphological, chemical, thermal, and mechanical properties of the solvent-extracted biopolymer were determined by various techniques (SEM, GC, 13C NMR, FT-IR, TGA, and tensile testing). The copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) consisted of 87 mol% β-hydroxybutyric acid (HB) and 13 mol% β-hydroxyvaleric acid (HV). With chemical properties similar to commercial PHBV and mechanical strength of around 30 MPa and 8% elongation at break, the biopolymer offers potential for industrial applications.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1080/1023666X.2010.500522
Publisher: Taylor & Francis
Keywords: Comamonas sp. EB 172; Characterization; Fed-batch fermentation; Mixed organic acids; PHBV
Depositing User: Nida Hidayati Ghazali
Date Deposited: 15 Jul 2013 01:58
Last Modified: 16 May 2017 09:56
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/1023666X.2010.500522
URI: http://psasir.upm.edu.my/id/eprint/16956
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