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Statistical optimization of the induction of the induction of phytase production by Arabinose in a recombinant E. coli using response surface methodology


Citation

Farouk, Abd-ElAziem and Meor Hussin, Anis Shobirin and Greiner, Ralf and Ismail, Shareef Mohideen and Batcha, Mohamed Faizal and Mohd Nur Lubis, Hamadah (2009) Statistical optimization of the induction of the induction of phytase production by Arabinose in a recombinant E. coli using response surface methodology. Asean Journal on Science and Technology for Development, 26 (1). pp. 67-78. ISSN 0217-5460

Abstract

The production of phytase in a recombinant E.coli using the pBAD expression system was optimized using response surface methodology with full-factorial faced centered central composite design. The ampicilin and arabinose concentration in the cultivation media and the incubation temperature were optimized in order to maximize phytase production using 23 central composite experimental design. With this design the number of actual experiment performed could be reduced while allowing eludidation of possible interactions among these factors. The most significant parameter was shown to be the linear and quadratic effect of the incubation temperature. Optimal conditions for phytase production were determined to be 100 µg/ml ampicilin, 0.2 % arabinose and an incubation temperature of 37ºC. The production of phytase in the recombinant E. coli was scaled up to 100 ml and 1000 ml.


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Additional Metadata

Item Type: Article
Subject: Phytases.
Subject: Escherichia coli.
Divisions: Faculty of Food Science and Technology
Publisher: ThaiScience
Keywords: Recombinant phytase; Statistical optimization; Cultivation conditions.
Depositing User: Khairil Ridzuan Khahirullah
Date Deposited: 14 Nov 2012 07:49
Last Modified: 05 Oct 2015 07:29
URI: http://psasir.upm.edu.my/id/eprint/16840
Statistic Details: View Download Statistic

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