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Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR.


Citation

Chai, Fung Pui and Woan, Chwen Wong and Chai, Lay Ching and Nillian, Elexson and Mohamad Ghazali, Farinazleen and Cheah, Yoke Kqueen and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Radu, Son (2011) Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR. Food Control, 22 (2). pp. 337-342. ISSN 0956-7135

Abstract / Synopsis

This study aimed to determine the prevalence and quantity of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits from hawker stalls and hypermarkets in Malaysia. Analysis was carried out using the most probable number (MPN) – multiplex polymerase chain reaction (PCR) method. The prevalence of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in 210 samples of sliced fruits examined were 23.3%, 7.6% and 3.8%, respectively with estimated quantity varying from 0 to 19 MPN/g. This study urged the authority to look into the biosafety of sliced fruits in Malaysia.


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Additional Metadata

Item Type: Article
Subject: Salmonella food poisoning
Subject: Foodborne diseases - Prevention
Subject: Food - Microbiology
Divisions: Faculty of Food Science and Technology
DOI Number: https://doi.org/10.1016/j.foodcont.2010.05.021
Publisher: Elsevier
Keywords: Salmonella; Sliced fruits; Multiplex PCR; Simultaneous detection.
Depositing User: Khairil Ridzuan Khahirullah
Date Deposited: 24 May 2012 03:15
Last Modified: 16 Oct 2015 08:47
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.foodcont.2010.05.021
URI: http://psasir.upm.edu.my/id/eprint/16726
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