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Sequence analysis and characterization of vacuolar-type H+-ATPase proteolipid transcript from Acanthus ebracteatus Vahl


Citation

Ho, Chai Ling and Nguyen, Phuoc Dang and Harikrishna, Jennifer Ann and Abdul Rahim, Raha (2008) Sequence analysis and characterization of vacuolar-type H+-ATPase proteolipid transcript from Acanthus ebracteatus Vahl. DNA Sequence, 19 (1). pp. 73-77. ISSN 1042-5179; ESSN: 1029-2365

Abstract

The vacuolar-type H+-ATPase (V-ATPase) is a multimeric enzyme with diverse functions in plants such as nutrient transport, flowering, stress tolerance, guard cell movement and development. A partial sequence of V-ATPase proteolipid was identified among the expressed sequence tags (ESTs) generated from Acanthus ebracteatus, and selected for full-length sequencing. The 876-nucleotide cDNA consists of an open reading frame of 165 amino acids. The deduced amino acid sequence displays high similarity (81%) with its homologs from Arabidopsis thaliana, Avecinnia marina and Gossypium hirsutum with the four transmembrane domains characteristics of the 16 kDa proteolipid subunit c of V-ATPase well conserved in this protein. Southern analysis revealed the existence of several members of proteolipid subunit c of V-ATPase in A. ebracteatus. The mRNA of this gene was detected in leaf, floral, stem and root tissues, however, the expression level was lower in stem and root tissues.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1080/10425170701445501
Publisher: Informa UK
Keywords: Acanthus ebracteatus; Mangrove; Sequence; Transcript; Vacuolar-type H+-ATPase proteolipid
Depositing User: Nida Hidayati Ghazali
Date Deposited: 16 Jul 2012 03:09
Last Modified: 22 Oct 2019 02:20
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/10425170701445501
URI: http://psasir.upm.edu.my/id/eprint/16672
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