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Scopariadulcis (SDF7) endowed with glucose uptake properties on L6 myotubes compared insulin.


Joo, Ee Beh and Latip, Jalifah and Abdullah, Mohd Puad and Ismail, Amin and Hamid, Muhajir (2010) Scopariadulcis (SDF7) endowed with glucose uptake properties on L6 myotubes compared insulin. Journal of Ethnopharmacology, 129 (1). pp. 23-33. ISSN 0378-8741, ESSN: 1872-7573


Aim of the study Insulin stimulates glucoseuptake and promotes the translocation of glucose transporter 4 (Glut 4) to the plasma membrane on L6myotubes. The aim of this study is to investigate affect of Scopariadulcis Linn water extracts on glucoseuptake activity and the Glut 4 translocation components (i.e., IRS-1, PI 3-kinase, PKB/Akt2, PKC and TC 10) in L6myotubescompared to insulin. Materials and methods Extract from TLC fraction-7 (SDF7) was used in this study. The L6myotubes were treated by various concentrations of SDF7 (1 to 50 μg/ml) and insulin (1 to 100 nM). The glucoseuptake activities of L6myotubes were evaluated using 2-Deoxy-D-glucoseuptake assay in with or without fatty acid-induced medium. The Glut 4 translocation components in SDF7-treated L6myotubes were detected using immunoblotting and quantified by densitometry compared to insulin. Plasma membrane lawn assay and glycogen colorimetry assay were carried out in SDF7- and insulin-treated L6myotubes in this study. Results Here, our data clearly shows that SDF7 possesses glucoseuptakeproperties on L6myotubes that are dose-dependent, time-dependent and plasma membrane Glut 4 expression-dependent. SDF7 successfully stimulates glucoseuptake activity as potent as insulin at a maximum concentration of 50 μg/ml at 480 min on L6myotubes. Furthermore, SDF7 stimulates increased Glut 4 expression and translocation to plasma membranes at equivalent times. Even in the insulin resistance stage (free fatty acids-induced), SDF7-treated L6myotubes were found to be more capable at glucose transport than insulin treatment. Conclusions Thus, we suggested that Scopariadulcis has the potential to be categorized as a hypoglycemic medicinal plant based on its good glucose transport properties.

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Additional Metadata

Item Type: Article
Subject: Medicinal plants.
Subject: Plants - Identification.
Subject: Insulin resistance.
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1016/j.jep.2010.02.009
Publisher: Elsevier
Keywords: Scopariadulcis; Insulin; Insulin resistance; Glucose transport; Glut 4.
Depositing User: Raja Norazlinda Raja Azenam
Date Deposited: 14 May 2013 07:00
Last Modified: 07 Oct 2015 07:49
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.jep.2010.02.009
URI: http://psasir.upm.edu.my/id/eprint/16629
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