UPM Institutional Repository

Production of reducing sugars by Trichoderma sp. KUPM0001 during solid substrate fermentation of sago starch processing waste hampas


Citation

Shahrim, Zaizuhana and Sabaratnam, Vikineswary and Abdul Rahman, Nor'aini and Abd. Aziz, Suraini and Hassan, Mohd Ali and Abdul Karim, Mohamed Ismail (2008) Production of reducing sugars by Trichoderma sp. KUPM0001 during solid substrate fermentation of sago starch processing waste hampas. Research Journal of Microbiology, 3 (9). pp. 569-579. ISSN 1816-4935

Abstract

Trichoderma sp. KUPM0001 showed good growth during solid substrate fermentation (SSF)of sagopith residue known as hampas, supplemented with 10% (v/w) of mineral salts solution containing 0.5% (w/v) (83.3mM)urea as nitrogen source and an initial moisture content of 80% (v/w). Mycelium suspension of 10% (v/w) density was used as initial inoculum and SSF was carried out at 25±2°C in static condition over a period of 120h. The parameters optimized include the initial moisture content of the substrate, mineral salts solution, urea concentration, inoculum density, incubation temperature and incubation time. Without optimized condition, the maximum reducing sugar obtained was 24mg mL¯¹ compared to 46 mg mL¯¹ substrate during optimized SSF after 96h incubation. The optimum parameters obtained were 80% (v/w) of initial moisture; 10% (v/w) of inoculums size; 1.0% of urea in 20% (w/v) of mineral solution and incubated at 30±2°C. The enzyme activities using optimized condition gave maximum α-amylase, glucoamylase, carboxymethyl cellulase, filter paperase and β-glucosidase of 3.19, 2.22, 1.66, 1.11 and 1.48 U mL¯¹, respectively.


Download File

[img]
Preview
PDF (Abstract)
Production of reducing sugars by Trichoderma sp. KUPM0001 during solid substrate fermentation of sago starch processing waste hampas.pdf

Download (50kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.3923/jm.2008.569.579
Publisher: Academic Journals
Keywords: Trichoderma sp. KUPM0001; Optimization; Solid substrate fermentation
Depositing User: Nida Hidayati Ghazali
Date Deposited: 12 Feb 2014 07:47
Last Modified: 02 Sep 2016 07:27
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3923/jm.2008.569.579
URI: http://psasir.upm.edu.my/id/eprint/16363
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item