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Production of mannan-degrading enzymes from Aspergillus niger and Sclerotium rolfsii using palm kernel cake as carbon source.


Citation

Abd. Aziz, Suraini and Ab Razak, Nor Asma and Musa, Mohamed Hanafi and Hassan, Mohd Ali (2009) Production of mannan-degrading enzymes from Aspergillus niger and Sclerotium rolfsii using palm kernel cake as carbon source. Research Journal of Agriculture and Biological Sciences, 3 (2). pp. 251-256. ISSN 1819-3412

Abstract

The aim of this study is to produce and profile the mannan-degrading enzymes from local fungal isolates by submerged fermentation and saccharification of PKC. Results showed that Aspergillus niger and Sclerotium rolfsii can produce mannan-degrading enzymes. The fungi were grown in submerged fermentation of PKC to produce mannan-degrading enzymes. The highest alpha-galactosidase was obtained on day 13 of fermentation (0.128 U mL-1) when using A. niger and on day 18 (0.126 U mL-1) when using S. rolfsii. Analysis also showed that enzyme activities for beta-mannanase using S. rolfsii were the highest at day 17 (3.166 U mL-1) and for A. niger (2.482 U mL-1) at day 8. Meanwhile the highest beta-mannosidase were obtained at day 16 for A. niger (0.128 U mL-1) and for S. rolfsii at day 16 (0.116 U mL-1).


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.3923/rjes.2009.251.256
Publisher: Academic Journals Inc.
Keywords: Mannan-degrading enzymes; Aspergillus niger; Sclerotium rolfsii; Palm kernel cake (PKC); Carbon source.
Depositing User: Nida Hidayati Ghazali
Date Deposited: 12 Feb 2014 07:37
Last Modified: 26 Nov 2015 07:05
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3923/rjes.2009.251.256
URI: http://psasir.upm.edu.my/id/eprint/16360
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