UPM Institutional Repository

Preparation and characterisation of protein concentrates from defatted kenaf seed.


Mariod , Abdalbasit Adam and Fathy, Siti Farhana and Saleem Ismail, Maznah (2010) Preparation and characterisation of protein concentrates from defatted kenaf seed. Food Chemistry, 123 (3). pp. 747-752. ISSN 0308-8146


Two kenaf varieties QP3 and V36 were used to obtain protein concentrates. Proximate analysis, foaming, water and oil absorption properties were studied. Significant (P<0.05) differences were observed among the two varieties only in their content in oil and carbohydrates. The protein concentrate yield was 13.04% and 10.56%, respectively. The two varieties showed significantly different (P<0.05) water and oil absorption capacities. QP3 showed higher foaming capacity than did V38, and it was increased with increasing salt and sugar concentration. Albumin was the main fraction representing 59.6% and 66.1% in QP3 and V36 varieties, respectively, followed by globulin, which represented 22.6% and 19.1%, respectively. The ratios of albumin, globulin, glutelin and prolamin were significantly different. Based on the data obtained from sodium dodecyl sulphate polyacrylamide gel electrophoresis, the main kenaf seed proteins present in the concentrates were five proteins with molecular weights ranging from 10 to 66. kDa. From differential scanning calorimetry data, QP3 and V36 protein concentrates had similar denaturation temperatures (82.6 and 81.8°C, respectively).

Download File

PDF (Abstract)
Preparation and characterisation of protein concentrates from defatted kenaf seed.pdf

Download (84kB) | Preview
Official URL or Download Paper: http://www.elsevier.com/

Additional Metadata

Item Type: Article
Subject: Kenaf.
Subject: Crops - Seeds - Proteins.
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1016/j.foodchem.2010.05.045
Publisher: Elsevier
Keywords: Differential scanning calorimetry; Foaming property; Hibiscus cannabinus; Oil absorption; Protein concentrate; Sodium dodecyl sulphatepolyacrylamide gel electrophoresis; Water absorption.
Depositing User: Raja Norazlinda Raja Azenam
Date Deposited: 16 May 2013 03:43
Last Modified: 20 Oct 2015 07:25
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.foodchem.2010.05.045
URI: http://psasir.upm.edu.my/id/eprint/16305
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item