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Phenotypic and molecular identification of a novel thermophilic Anoxybacillus species: a lipase-producing bacterium isolated from a Malaysian hotspring.


Citation

Olusesan, Akanbi Taiwo and Kamaruzaman, Liana Azura and Abu Bakar, Fatimah and N., Sheikh Abdul Hamid and Radu, Son and Saari, Nazamid (2009) Phenotypic and molecular identification of a novel thermophilic Anoxybacillus species: a lipase-producing bacterium isolated from a Malaysian hotspring. World Journal of Microbiology and Biotechnology, 25 (11). pp. 1981-1988. ISSN 0959-3993

Abstract / Synopsis

Eleven lipase-producing thermophilic bacteria strains were recently isolated from Kuala Woh Hot Spring, in Peninsular Malaysia. These strains have been qualitatively screened using Rhodamine B-olive oil plate agar. All strains showed lipase activity in the range of 0.56–2.62 U/ml. Their thermostabilities were then determined by incubation at 80°C for 30 min. Results showed that strain KW 6 and KW 12 produced relatively thermostable lipases, which retained 62 and 54% of their original activity, respectively. They were identified based on their morphological characteristics, biochemical tests and the Biolog system. Strain KW 12 showed exceptionally unique characteristics (over KW 6) being able to grow in a broad range of pH and temperature. It was further identified using 16S rRNA partial sequence analysis and the result of 16S rRNA partial sequence analysis identified KW 12 as Anoxybacillus kamchatkensis.


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Additional Metadata

Item Type: Article
Subject: Lipase.
Subject: Thermophilic bacteria.
Subject: Microorganisms - Detection.
Divisions: Faculty of Food Science and Technology
DOI Number: https://doi.org/10.1007/s11274-009-0097-0
Publisher: Springer Verlag
Keywords: Anoxybacillus kamchatkensis; Lipase activity; Thermophylic lipase and thermophilic bacteria.
Depositing User: Khairil Ridzuan Khahirullah
Date Deposited: 27 Aug 2012 04:14
Last Modified: 27 Aug 2012 04:14
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s11274-009-0097-0
URI: http://psasir.upm.edu.my/id/eprint/16181
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