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Optimization of lipase-catalyzed synthesis of palm amino acid surfactant using response surface methodology (RSM)


Citation

Bidin, Hasmah and Basri, Mahiran and Mat Radzi, Salina and Ariff, Arbakariya and Raja Abdul Rahman, Raja Noor Zaliha and Salleh, Abu Bakar (2009) Optimization of lipase-catalyzed synthesis of palm amino acid surfactant using response surface methodology (RSM). Industrial Crops and Products, 30 (2). pp. 206-211. ISSN 0926-6690; ESSN: 1872-633X

Abstract / Synopsis

Amino acid surfactants are high-value surfactants which have excellent emulsifying characteristics and minimal toxicity to the living body. Enzymatic synthesis of palm kernel amino acid surfactant was optimized by response surface methodology (RSM) using palm kernel olein (PKO) and l(+)-lysine catalyzed by Lipozyme RM IM. The reaction was performed in batch mode stirred tank reactor (STR) with one multi-bladed impeller. A central composite rotatable design (CCRD) was employed to evaluate the interactive effects of various parameters. The parameters were temperature (A): (40.00–70.00 °C), impeller speed (B): (100.00–400.00 rpm), substrates ratio (C): (1.00–4.00 mmol) and enzyme amount (D): (5.00–8.00 g). The optimum condition derived via RSM at fixed reaction time of 24 h was temperature; 47.50 °C, impeller speed; 323.96 rpm, substrates ratio; 3.25 mmol and enzyme amount; 7.25 g. The experimental yield was 89.03% under the optimum condition, which compared well with the maximum predicted value of 93.77%.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
DOI Number: https://doi.org/10.1016/j.indcrop.2009.03.006
Publisher: Elsevier
Keywords: Aminoacid surfactants; Palm kernel olein (PKO); Lipozyme; Stirred tank reactor (STR); Central composite rotatable design (CCRD)
Depositing User: Nida Hidayati Ghazali
Date Deposited: 01 Aug 2012 00:44
Last Modified: 27 Sep 2016 08:07
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.indcrop.2009.03.006
URI: http://psasir.upm.edu.my/id/eprint/15974
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