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Mo (VI) reduction to molybdenum blue by Serratia marcescens strain Dr. Y9.


Abd. Shukor, Mohd. Yunus and Hamdan Mustafa, Mohd Hamim and Othaman, Muhammad Anas and Shamaan, Nor Aripin and Syed, Mohd. Arif (2009) Mo (VI) reduction to molybdenum blue by Serratia marcescens strain Dr. Y9. Polish Journal of Microbiology, 58 (2). pp. 141-147. ISSN 1733-1331


In this work we report on the isolation of a local molybdenum-reducing bacterium. The bacterium reduced molybdate or Mo(6+) to molybdenum blue (oxidation states between 5+ to 6+). Electron donors that supported cellular growth were sucrose, maltose, mannitol,fructose, glucose and starch (in decreasing order) with sucrose supporting formation of the highest amount of molybdenum blue at 10 g/l after 24 hours of static incubation. The optimum molybdate and phosphate concentrations that supported molybdate reduction were 20 and 5 mM, respectively. Molybdate reduction was optimal at 37∞C. The molybdenum blue produced from cellular reduction exhibited a unique absorption spectrum with a maximum peak at 865 nm and a shoulder at 700 nm. The isolate was tentatively identified as S. marcescens strain Dr.Y9 based on carbon utilization profiles using Biolog GN plates and partial 16S rDNA molecular phylogeny. No inhibition of molybdenum-reducing activity was seen using electron transport system (ETS) inhibitors such as antimycin A,1 HQNO (Hydro-xyquinoline-N-Oxide), sodium azide and cyanide suggesting that the ETS of this bacterium is not the site of molybdate reduction.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Food Science and Technology
Publisher: Polish Society of Microbiologist
Keywords: Serratia marcescens; Molybdate-reduction; Molybdenum blue.
Depositing User: Nida Hidayati Ghazali
Date Deposited: 06 Mar 2014 07:45
Last Modified: 24 Nov 2015 08:03
URI: http://psasir.upm.edu.my/id/eprint/15652
Statistic Details: View Download Statistic

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