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Mitochondrial DNA diversity of Tor douronensis Valenciennes (Cyprinidae) from five natural populations in Malaysia


Esa, Yuzine and Siraj, Siti Shapor and Daud, Siti Khalijah and A. Rahim, Khairul Adha and Japning, Jeffrine Rovie Ryan and Tan, Soon Guan (2008) Mitochondrial DNA diversity of Tor douronensis Valenciennes (Cyprinidae) from five natural populations in Malaysia. Zoological Studies, 47 (3). pp. 360-367. ISSN 1021-5506


In this study, we examined the genetic structure of Tor tambroides Valenciennes, an important indigenous freshwater fish species in Malaysia, using sequence analysis of 464 base pairs of the mitochondrial cytochrome c oxidase I(COI) gene. In total, 92 T. tambroides samples were analyzed from 4 locations on Peninsular Malaysia (n=87) and a single population from Sarawak (Batang Ai, n=5) on Borneo Island, and 4 sequences of Tor douronensis from Sarawak were used for comparisons. In total, 9 haplotypes were found, with 7 haplotypes being unique and 2 haplotypes being shared among the 5 populations. The phylogenetic analysis using Neighbor-joining (NJ) and maximum-parsimony (MP) methods supported the monophyletic status between T. tambroides and T. douronensis, thus suggesting their status as different species. The clustering of all T. tambroides samples into a single clade suggested that their genetic identity belongs to a single species. The sharing of haplotype HKE4 between Batang Ai of Sarawak (n= 4) and Perak of Peninsular Malaysia (n= 3) reflects the historical connection of drainages between the regions possibly during Pleistocene glaciation periods. Limited variations were found among all peninsular T. tambroides populations. The low level of mitochondrial (mt)DNA differences currently found among T. tambroides populations is probably due to the high proportion of the HKE1 haplotype being found in all the populations (0.736-1.000), or the small number of samples used in the present study. Overall, the present study was able to shed light on the phylogenetic relationships and genetic structure of T. tambroides in Malaysia.

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Official URL or Download Paper: http://zoolstud.sinica.edu.tw/473.htm

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Publisher: Biodiversity Research Center, Academia Sinica, Taiwan
Keywords: Tor tambroides; Freshwater fish; mtDNA COI; Population structure
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 01 Aug 2015 04:54
Last Modified: 16 May 2016 05:03
URI: http://psasir.upm.edu.my/id/eprint/15645
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