Meat species identification and Halal authentication analysis using mitochondrial DNA.

Citation

Murugaiah, Chandrika and Mohd Noor, Zainon and Mastakim, Maimunah and Bilung, Lesley Maurice and Selamat, Jinap and Radu, Son (2009) Meat species identification and Halal authentication analysis using mitochondrial DNA. Meat Science, 83 (1). pp. 57-61. ISSN 0309-1740

Abstract

A method utilizing PCR-restriction fragment length polymorphism (RFLP) in the mitochondrial genes was developed for beef (Bos taurus), pork (Sus scrofa), buffalo (Bubalus bubali), quail (Coturnix coturnix), chicken (Gallus gallus), goat (Capra hircus), rabbit (Oryctolagus cuniculus) species identification and Halal authentication. PCR products of 359-bp were successfully obtained from the cyt b gene of these six meats. AluI, BsaJI, RsaI, MseI, and BstUI enzymes were identified as potential restriction endonucleases to differentiate the meats. The genetic differences within the cyt b gene among the meat were successfully confirmed by PCR-RFLP. A reliable typing scheme of species which revealed the genetic differences among the species was developed.

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Additional Metadata

Item Type:	Article
Subject:	Mitochondrial DNA.
Subject:	Halal food.
Divisions:	Faculty of Food Science and Technology
DOI Number:	https://doi.org/10.1016/j.meatsci.2009.03.015
Publisher:	Elsevier
Keywords:	Cyt b gene; Mitochondrial DNA; Halal authentication; Forensic science; Meat; PCR-restriction fragment length polymorphism (RFLP); Species identification.
Depositing User:	Khairil Ridzuan Khahirullah
Date Deposited:	22 May 2013 07:45
Last Modified:	22 Oct 2015 23:35
Altmetrics:	http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.meatsci.2009.03.015
URI:	http://psasir.upm.edu.my/id/eprint/15580
Statistic Details:	View Download Statistic

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