UPM Institutional Repository

Live recombinant Lactococcus lactis vaccine expressing aerolysin genes D1 and D4 for protection against Aeromonas hydrophila in tilapia (Oreochromis niloticus)


Citation

Anuradha, Kathragadda and Foo, Hooi Ling and Nor Shamsudin, Mariana and Loh, Teck Chwen and Yusoff, Khatijah and Mohd Daud, Hassan and Sasan, Hosseinali and Abdul Rahim, Raha (2010) Live recombinant Lactococcus lactis vaccine expressing aerolysin genes D1 and D4 for protection against Aeromonas hydrophila in tilapia (Oreochromis niloticus). Journal of Applied Microbiology, 109 (5). pp. 1632-1642. ISSN 1364-5072; ESSN: 1365-2672

Abstract

Aims:  To evaluate a live recombinant Lactococcus lactis vaccine expressing aerolysin genes D1 (Lac-D1ae) and/or D4 (Lac-D4ae) in protection against Aeromonas hydrophila in tilapia (Oreochromis niloticus). Methods and Results:  The polymerase chain reaction (PCR)-amplified 250- and 750-bp sequences coding for domains D1 and D4 of aerolysin were individually cloned into pNZ8048 and electrotransformed into L. lactis. The recombinant vaccine candidates were then either orally fed or injected intraperitoneally into tilapia. The development of antibodies in sampled fish compared to control groups implied that the recombinant epitopes expressed in L. lactis were able to elicit an immunogenic response in tilapia. Interestingly, the lower doses of both Lac-D1ae and Lac-D4ae gave higher antibody levels over the study period. Fish immunized with Lac-D1ae and Lac-D4ae together showed the highest level of protection, and the mortality was reduced significantly compared to control strains in both modes of vaccination. Conclusions:  The recombinant L. lactis strain expressing D1 and D4 produced aerolysin-specific serum IgM in tilapia. Both D1 and D4 promoted 55–82% relative per cent survival (RPS) against Aeromonas infection through intraperitoneal injection, whereas the RPS following oral feeding of the vaccine was 70–100%. Significance and Impact of the Study:  The D1 and D4 regions of the aerolysin protein have been successfully identified as immunogenic regions that can elicit antibody production in tilapia and protect against challenge with Aer. hydrophila. A promising oral vaccine using L. lactis harbouring the D1 and D4 regions has been developed to control Aer. hydrophila.


Download File

[img]
Preview
PDF (Abstract)
Live recombinant Lactococcus lactis vaccine expressing aerolysin genes D1 and D4 for protection against Aeromonas hydrophila in tilapia.pdf

Download (185kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Agriculture
Faculty of Medicine and Health Science
Faculty of Veterinary Medicine
Institute of Bioscience
DOI Number: https://doi.org/10.1111/j.1365-2672.2010.04789.x
Publisher: The Society for Applied Microbiology
Keywords: Aerolysin; Aeromonas hydrophila; Lactococcus lactis; Recombinant vaccine; Tilapia
Depositing User: Nida Hidayati Ghazali
Date Deposited: 24 Jul 2012 07:57
Last Modified: 22 Oct 2015 03:53
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1111/j.1365-2672.2010.04789.x
URI: http://psasir.upm.edu.my/id/eprint/15473
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item