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Interfacial partition of plasmid DNA in aqueous two-phase systems


Luechau, Frank and Ling, Tau Chuan and Lyddiatt, Andrew (2009) Interfacial partition of plasmid DNA in aqueous two-phase systems. Separation and Purification Technology, 67 (1). pp. 64-70. ISSN 1383-5866; ESSN: 1873-3794


In this paper, the partition of plasmid DNA (pDNA) in polyethylene glycol (PEG) 300 and di-potassium hydrogen phosphate aqueous two-phase systems (ATPS) under gravity separation was investigated. The influence of temperature (20 and 30 °C) and mode of phase separation (40–60% (w/w) lysate concentrations) on the pDNA partition behaviour was studied. At 20 °C, 70.7% of pDNA partitioned to the top phase while the recoveries in the interface and bottom phase were comparatively small. At 30 °C, the partition had switched from a top phase preference to an interface preference where 73.9% of pDNA was recovered. The distribution of pDNA between the top phase and interface is effectively time-dependent during gravitational phase separation. Systems with lysate concentrations between 40 and 50% (w/w) separated with a continuous bottom phase and a dispersed PEG-rich top phase. For ATPS with 55 and 60% (w/w) lysate, the top phase was continuous and the bottom phase was dispersed.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Engineering
DOI Number: https://doi.org/10.1016/j.seppur.2009.03.027
Publisher: Elsevier
Keywords: Interfacial partition; Plasmid DNA; Aqueous two-phase systems; Top phase; Bottom phase
Depositing User: Nabilah Mustapa
Date Deposited: 09 May 2019 01:28
Last Modified: 09 May 2019 01:28
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.seppur.2009.03.027
URI: http://psasir.upm.edu.my/id/eprint/15267
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