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Incidence of extended spectrum beta-lactamase producing pseudomonas aeruginosa and frequency of oxa-2 and oxa-10 genes.


Citation

Alipour, Tanaz and Sadeghifard, Nourkhoda and Amirmozafari, Nour and Ghafurian, Sobhan and Abdulamir, Ahmed Sahib and Mohebi, Reza and Abu Bakar, Fatimah and Raftari, Mohammad (2010) Incidence of extended spectrum beta-lactamase producing pseudomonas aeruginosa and frequency of oxa-2 and oxa-10 genes. Australian Journal of Basic and Applied Sciences, 4 (8). pp. 3202-3207. ISSN 1991-8178

Abstract

Pseudomonas aeruginosa has emerged as a major cause of infection in the last few decades. The objective of this study was to determine the distribution of extended spectrum beta lactamaseproducing P. aeruginosa and the frequency of OXA-2 and OXA-10 genes. 350 and 120 P. aeruginosa samples were isolated from patients in two locations in Iran representing the region of the Middle East. Antibiotic pattern and OXA type ESBLs were detected by disk diffusion and PCR methods. Of 350 isolates of P. aeruginosa isolates in the first location, 105 (30%) isolates were positive for ESBLs from which 92 (87.61%) isolates were positive for OXA-10 Type ESBLs while only 5 (4.76%) isolates were positive for OXA-2 ESBLs by using PCR methods in Ilam hospitals. In the second location, 46 (38.33%) of P. aeruginosa isolates were positive for ESBLs of which 29 (63%) were positive for OXA-10 Type ESBLs and only 1 (2.1%) isolates were positive for OXA-2 ESBLs by PCR methods in Kerman hospitals. Results of the current study showed the presence of high percentage of OXA genes, 2 and 10, ESBLs in the region of the Middle East associated with extended resistance profile against almost all cephalosporins. Moreover, comparing the results of the studied two cities indicated that distribution of OXA type ESBLs is locally diverse even in the same geographical region.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Publisher: AJBAS Journal
Keywords: Pseudomonas aeruginosa; OXA-2; OXA-10; Gene; ESBLs.
Depositing User: Khairil Ridzuan Khahirullah
Date Deposited: 23 May 2013 01:34
Last Modified: 02 Nov 2015 03:40
URI: http://psasir.upm.edu.my/id/eprint/15178
Statistic Details: View Download Statistic

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