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Incidence and characterization of Salmonella species in street food and clinical samples


Robin, Tunung and Chai, Lay Ching and Ragavan, Usha Menon and Bilung, Lesley Maurice and Cheah, Yoke Kqueen and Patrick, Guda Benjamin and Mohamad Ghazali, Farinazleen and Abu Bakar, Fatimah and Malakar, Pradeep Kumar and Radu, Son (2007) Incidence and characterization of Salmonella species in street food and clinical samples. Journal of Food Safety, 27 (4). pp. 345-361. ISSN 0149-6085; ESSN: 1745-4565


The objectives of our study were to investigate the Salmonella species contamination in various types of ready-to-eat street-vended dishes or drinks, to isolate Salmonella spp. from clinical samples and to assess the possible relationship between the serotypes isolated from these two different environments. The isolates were characterized by their antibiotic resistance, plasmid profiles and randomly amplified polymorphic DNA (RAPD) sequences. A total of 24 salmonellae, belonging to seven different serotypes, were isolated from 129 different street-vended foods and drinks and 12 clinical samples (rectal swabs). The encountered serotypes from street foods were Salmonella Biafra (n = 8), Salmonella Braenderup (n = 3) and Salmonella Weltevreden (n = 1), and from clinical samples were Salmonella Typhi (n = 8), Salmonella Typhimurium (n = 2), Salmonella Paratyphi A (n = 1) and Salmonella Paratyphi B (n = 1). The results showed no similarities in the types of Salmonella serotypes from street food and clinical samples examined. The Salmonella strains were resistant to one or more of the 14 tested antibiotics. Seventeen isolates harbored plasmids, with plasmid sizes ranging from 3.0 to 38.5 MDa. RAPD fingerprinting with primers OPAR3 and OPAR8 produced a combination of 21 fingerprint patterns. The dendrograms generated for the S. Biafra and S. Typhi showed strains of the same serotypes but of very different types of sample and location of sampling clustered together, indicating the possibility of cross contamination during food handling.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
DOI Number: https://doi.org/10.1111/j.1745-4565.2007.00089.x
Publisher: Blackwell Publishing
Keywords: Salmonella; Street food; Clinical samples
Depositing User: Khairil Ridzuan Khahirullah
Date Deposited: 23 May 2013 01:42
Last Modified: 15 Nov 2016 03:05
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1111/j.1745-4565.2007.00089.x
URI: http://psasir.upm.edu.my/id/eprint/15177
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