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In vitro culture of Borneo's endemic orchid, Vanda dearei


Citation

Jawan, Roslina and Gansau, Jualang @ Azlan Abdullah and Abdullah, Janna Ong (2010) In vitro culture of Borneo's endemic orchid, Vanda dearei. Asia Pacific Journal of Molecular Biology and Biotechnology, 18 (1). pp. 203-207. ISSN 0128-7451

Abstract

The effect of basal media, complex additives and sugars to the growth and development of V. dearei protocorms were reported in this study. Protocorms were initiated from in vitro seeds germination from immature capsule. Among the three basal media, growth of protocorms was superior in ½ MS medium followed by KC and VW with maximum percentage of protocorms with leaf and root, and mean number of leaf and root produced. Meanwhile, 0.2% (w/v) yeast extract markedly enhanced the growth of protocorms. In sugar study, results show that protocorms preferred sucrose as compared to fructose and glucose. The leaf and root formation was significantly enhanced as the sugar concentration was decreased. Medium supplemented with 2%(w/v) sucrose was the best compared to the other treatments and sugar at a concentration of 4% (w/v) induced the formation of large size seedlings.


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Official URL or Download Paper: http://www.msmbb.org.my/apjmbb/html181/181av.htm

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Publisher: Malaysian Society for Molecular Biology and Biotechnology
Notes: Proceedings Asia Pacific Conference on Plant Tissue and Agribiotechnology (APaCPA) 17-21 June 2007
Keywords: Vanda dearei; Protocorms; Propagation; Growth medium
Depositing User: Ms. Nida Hidayati Ghazali
Date Deposited: 24 Jul 2013 02:54
Last Modified: 19 Jan 2018 08:16
URI: http://psasir.upm.edu.my/id/eprint/15162
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