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In vitro antimicrobial activity of Muntingia calabura extracts and fractions


Zakaria, Zainul Amiruddin and Sufian, Adila Shahida and Ramasamy, Kalavathy and Ahmat, Norizan and Sulaiman, Mohd Roslan and Abdul Kadir, Arifah and Ahmad, Zuraini and Abdullah, Muhammad Nazrul Hakim (2010) In vitro antimicrobial activity of Muntingia calabura extracts and fractions. African Journal of Microbiology Research, 4 (4). art. no. 0FE895611797. pp. 304-308. ISSN 1996-0808


The aim of the present study is to determine the in vitro antimicrobial activity of various extracts, partitions and fractions of Muntingia calabura (Elaeocarpaceae) leaves against a selected panel of microorganisms. The leaves of M. calabura were soaked separately in the aqueous, chloroform and methanol solvent systems in the ratio of 1:20 (w/v) for 72 h and these procedures were repeated three times. Antimicrobial testing was carried out using the micro-broth dilution method. The microbes targeted were Staphylococcus aureus 25923, S. aureus 33591 (a multi-drugs resistant S. aureus (MRSA) isolate), Escherichia coli 35218, Pseudomonas aeruginosa ATCC 27853, Candida albicans 10231 and Microsporum canis ATCC 36299. The methanol extract inhibited MSSA (MIC = 1250 μg/ml; MBC = 1250 μg/ml) and MRSA (MIC = 1250 μg/ml; MBC = 1250 μg /ml) and considered the most effective extract and was further partitioned sequentially using the aqueous, petroleum ether and ethyl acetate. The ethyl acetate partition exhibited effective antibacterial activities with the MIC/MBC value of 156 and 313 μg/ml against S. aureus 25923 and S. aureus 33591, respectively. The ethyl acetate partition underwent fractionation process and yielded 15 fractions (A1-A15) of which only fractions A9 to A15 effectively inhibited the growth of S. aureus 25923 and S. aureus 33591 with MIC/MBC values ranging from 78 to 2500 μg/ml.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Faculty of Veterinary Medicine
Publisher: Academic Journals
Keywords: Antimicrobial activity; Elaeocarpaceae; Ethyl acetate fraction; Micro-broth dilution; Muntingia calabura
Depositing User: Raja Norazlinda Raja Azenam
Date Deposited: 17 Oct 2013 04:14
Last Modified: 20 Nov 2017 03:20
URI: http://psasir.upm.edu.my/id/eprint/15159
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