Citation
Wong, Sing King and Tan, Wen Siang and Omar, Abdul Rahman and Tan, Chon Seng and Yusoff, Khatijah
(2009)
Immunogenic properties of recombinant ectodomain of Newcastle disease virus hemagglutinin-neuraminidase protein expressed in Escherichia coli.
Acta Virologica, 53 (1).
pp. 35-41.
ISSN 0001-723X
Abstract
Hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) plays a vital role in the viral infectivity, host immunity, and disease diagnosis. A portion of the HN gene encoding the ectodomain (nt 142-1739) was cloned and expressed in Escherichia coli yielding an insoluble HN protein and a soluble NusA-HN protein containing N-utilization substance A (NusA) fusion component. Both recombinant proteins were purified and used for immunization of chickens. The recombinant HN protein induced higher antibody titers as compared to the recombinant NusA-HN protein. These antibodies were able to react in immunoblot analysis with the corresponding recombinant proteins as well as with the HN protein of NDV.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Biotechnology and Biomolecular Sciences Faculty of Veterinary Medicine Institute of Bioscience |
| DOI Number: | 10:4149/av-2009-01-35 |
| Publisher: | Academic Electronic Press Ltd. |
| Keywords: | Newcastle disease virus; Hemagglutinin-neuraminidase; Cloning; Expression; Immunogenicity. |
| Depositing User: | Norhazura Hamzah |
| Date Deposited: | 25 Oct 2013 00:50 |
| Last Modified: | 22 Oct 2015 01:00 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10:4149/av-2009-01-35 |
| URI: | http://psasir.upm.edu.my/id/eprint/15097 |
| Statistic Details: | View Download Statistic |
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