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HPLC and anti-inflammatory studies of the flavonoid rich chloroform extract fraction of Orthosiphon stamineus leaves.


Citation

Yam, Mun Fee and Lim, Vuanghao and Muhammad Salman, Ibrahim and Ameer, Omar Ziad and Ang, Lee Fung and Noersal , Rosidah and Abdulkarim, Muthanna Fawzy and Abdullah, Ghassan Zuhair and Basir, Rusliza and Sadikun, Amirin and Asmawil, Mohd Zaini (2010) HPLC and anti-inflammatory studies of the flavonoid rich chloroform extract fraction of Orthosiphon stamineus leaves. Molecules, 15 (6). pp. 4452-4466. ISSN 1420-3049; ESSN: 1420-3049

Abstract

The aim of the present study was to verify the anti-inflammatory activity of Orthosiphon stamineus leaf extracts and to identify the active compound(s) contributing to its anti-inflammatory activity using a developed HPLC method. Active chloroform extract of O. stamineus was fractionated into three fractions using a dry flash column chromatography method. These three fractions were investigated for anti-peritoneal capillary permeability, in vitro nitric oxide scavenging activity, anti-inflammatory and nitric oxide (NO) inhibition using carrageenan-induced hind paw edema method. The flavonoid rich chloroform extract fraction (CF2) [containing sinensetin (2.86% w/w), eupatorin (5.05% w/w) and 3'-hydroxy-5,6,7,4'-tetramethoxyflavone (1.101% w/w)], significantly reduced rat hind paw edema, NO and decreased dye leakage to peritoneal cavity at p < 0.05. IC50 of in vitro NO scavenging of CF2 was 0.3 mg/mL. These results suggest that the anti-inflammatory properties of these CF2 may possibly be due to the presence of flavonoid compounds capable of affecting the NO pathway.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.3390/molecules15064452
Publisher: MDPI
Keywords: 3'-Hydroxy-5,6,7,4'-tetramethoxyflavone; Anti-inflammatory activity; Eupatorin; Orthosiphon stamineus; Peritoneal capillary permeability; Sinensetin.
Depositing User: Raja Norazlinda Raja Azenam
Date Deposited: 17 Oct 2013 05:11
Last Modified: 30 Oct 2015 00:46
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/molecules15064452
URI: http://psasir.upm.edu.my/id/eprint/15041
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