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Highly thermostable extracellular lipase-producing Bacillus strain isolated from a Malaysian hotspring and identified using 16S rRNA gene sequencing


Citation

Akanbi, Taiwo Olusesan and Kamaruzaman, Azura Liana and Abu Bakar, Fatimah and Sheikh Abdul Hamid, Nazimah and Radu, Son and Abdul Manap, Mohd Yazid and Saari, Nazamid (2010) Highly thermostable extracellular lipase-producing Bacillus strain isolated from a Malaysian hotspring and identified using 16S rRNA gene sequencing. International Food Research Journal, 17 (1). pp. 45-53. ISSN 1985-4668; ESSN: 2231-7546

Abstract

The activities of lipase produced by five lipases-producing thermophilic bacteria strains (SY1, SY5, SY6, SY7 and SY9) isolated from Selayang Hot Spring in the western part of Peninsular Malaysia were analyzed and compared. SY7 and SY9 had considerably higher lipolytic activities than those of SY1, SY5 and SY6. Thermostabilities of lipase produced by all strains were determined after heating at 80°C for 30 minutes. Strain SY7 retained the highest lipolytic activity of 77%, while others had infinitesimally low thermostability (retaining less than 34% of their original activity) at the same temperature and time. SY7 was chosen for further characterization because it showed exceptionally high lipase activity and thermostability. It was identified as belonging to Bacillus species by the conventional Gram-staining technique, Biochemical tests and Biolog Microstation system. By using 16S rRNA gene sequencing, strain SY7 generated the same expected PCR product with molecular weight of 1500 base pair. It displayed 98% sequence similarity to Bacillus cereus strain J-1 16S rRNA gene partial sequence with accession number: AY305275 and has been deposited in the database of Genbank.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Publisher: Faculty of Food Science and Technology, Universiti Putra Malaysia
Keywords: Thermostable; Lipase; 16S rRNA; Bacillus strain
Depositing User: Khairil Ridzuan Khahirullah
Date Deposited: 07 Jun 2013 01:06
Last Modified: 29 Sep 2015 01:18
URI: http://psasir.upm.edu.my/id/eprint/15013
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