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Hexavalent molybdenum reduction to molybdenum blue by S. marcescens Strain Dr. Y6


Citation

Abd. Shukor, Mohd. Yunus and M. Habib, S. H. and Abdul Rahman, Mohd Fadhil and Jirangon, Hussain and A. Abdullah, M. P. and Shamaan, Nor Aripin and Syed, Mohd Arif (2008) Hexavalent molybdenum reduction to molybdenum blue by S. marcescens Strain Dr. Y6. Applied Biochemistry and Biotechnology, 149 (1). pp. 33-43. ISSN 0273-2289; ESSN: 1559-0291

Abstract

A molybdate-reducing bacterium has been locally isolated. The bacterium reduces molybdate or Mo(6+) to molybdenum blue (molybdate oxidation states of between 5+ and 6+). Different carbon sources such as acetate, formate, glycerol, citric acid, lactose, fructose, glucose, mannitol, tartarate, maltose, sucrose, and starch were used at an initial concentration of 0.2% (w/v) in low phosphate media to study their effect on the molybdate reduction efficiency of bacterium. All of the carbon sources supported cellular growth, but only sucrose, maltose, glucose, and glycerol (in decreasing order) supported molybdate reduction after 24 h of incubation. Optimum concentration of sucrose for molybdate reduction is 1.0% (w/v) after 24 h of static incubation. Ammonium sulfate, ammonium chloride, valine, OH-proline, glutamic acid, and alanine (in the order of decreasing efficiency) supported molybdate reduction with ammonium sulfate giving the highest amount of molybdenum blue after 24 h of incubation at 0.3% (w/v). The optimum molybdate concentration that supports molybdate reduction is between 15 and 25 mM. Molybdate reduction is optimum at 35 degrees C. Phosphate at concentrations higher than 5 mM strongly inhibits molybdate reduction. The molybdenum blue produced from cellular reduction exhibits a unique absorption spectrum with a maximum peak at 865 nm and a shoulder at 700 nm. The isolate was tentatively identified as Serratia marcescens Strain Dr.Y6 based on carbon utilization profiles using Biolog GN plates and partial 16s rDNA molecular phylogeny.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1007/s12010-008-8137-z
Publisher: Humana Press
Keywords: S. marcescens; Molybdate-reduction; Molybdenum blue
Depositing User: Nida Hidayati Ghazali
Date Deposited: 24 Jul 2013 02:19
Last Modified: 13 Jul 2017 01:52
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s12010-008-8137-z
URI: http://psasir.upm.edu.my/id/eprint/15000
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