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Evaluation of safe attenuated vibrio alginolyticus for oral vaccination of lates calcarifer against vibriosis.


Citation

Idris, Nurul H. and Abdullah Al-Haj, Nagi Ahmed and Shamsudin, Mariana Nor and Abdul Rahim, Raha (2009) Evaluation of safe attenuated vibrio alginolyticus for oral vaccination of lates calcarifer against vibriosis. Research Journal of Biological Sciences, 4 (4). pp. 509-513. ISSN 1815-8846

Abstract

Safe attenuation has been done on marine pathogen Vibrio alginolyticus using naturally acidified fructose against vibriosis. Attenuation was confirmed by injecting the attenuated bacterium into fish where the survival rate was 100% compared to 50% survival in fish injected with non-attenuated bacteria. The attenuated bacterium was then evaluated for oral vaccination of Lates calcarifer (Asian seabass). Fish were fed with fish pellet incorporated with attenuated and non-attenuated bacterium of V. alginolyticus for 30 days. They were measured for serum antibody production by conventional agglutination titer and also monitored for the fish weight gain to observe the health improvement. Vaccinated fish showed comparable increased in weight gain, 90% survival after challenge and significantly high antibody titer compared to other treatment and control.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
DOI Number: rjbsci.2009.509.513
Publisher: Medwell Publishing
Keywords: Vaccines; Vibrio alginolyticus; Lates calcarifer; Minimal inhibitory concentration; Polymerase chain reaction.
Depositing User: Nida Hidayati Ghazali
Date Deposited: 19 Jul 2013 08:42
Last Modified: 29 Sep 2015 07:45
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=rjbsci.2009.509.513
URI: http://psasir.upm.edu.my/id/eprint/14611
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