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Evaluation of DNA and RNA extraction methods.


Chin Han, Edwin Shiaw and Mohd Sidik, Shiran and Cheah, Yoke Kqueen and Tan, Geok Chin and Abdul Rahman, Sabariah (2010) Evaluation of DNA and RNA extraction methods. Medical Journal of Malaysia, 65 (2). pp. 133-137. ISSN 0300-5283


This study was done to evaluate various DNA and RNA extractions from archival FFPE tissues. A total of 30 FFPE blocks from the years of 2004 to 2006 were assessed with each modified and adapted method. Extraction protocols evaluated include the modified enzymatic extraction method (Method A), Chelex-100 extraction method (Method B), heat-induced retrieval in alkaline solution extraction method (Methods C and D) and one commercial FFPE DNA Extraction kit (Qiagen, Crawley, UK). For RNA extraction, 2 extraction protocols were evaluated including the enzymatic extraction method (Method 1), and Chelex-100 RNA extraction method (Method 2). Results show that the modified enzymatic extraction method (Method A) is an efficient DNA extraction protocol, while for RNA extraction, the enzymatic method (Method 1) and the Chelex-100 RNA extraction method (Method 2) are equally efficient RNA extraction protocols.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Publisher: Malaysian Medical Association.
Keywords: Crosslinking; DNA extraction; FFPE; RNA extraction.
Depositing User: Raja Norazlinda Raja Azenam
Date Deposited: 11 Nov 2013 03:34
Last Modified: 11 Nov 2013 03:34
URI: http://psasir.upm.edu.my/id/eprint/14600
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