UPM Institutional Repository

Estimation of 16S RNA gene copy number in several probiotic Lactobacillus strains isolated from the gastrointestinal tract of chicken.


Citation

Chin, Mei Lee and Chin, Chin Sieo and Abdullah, Norhani and Yin, Wan Ho (2008) Estimation of 16S RNA gene copy number in several probiotic Lactobacillus strains isolated from the gastrointestinal tract of chicken. FEMS Microbiology Letters, 287 (1). pp. 136-141. ISSN 0378-1097

Abstract

The copy numbers of 16S rRNA genes in 12 probiotic Lactobacillus strains of poultry origin were analyzed. Genomic DNA of the strains was digested with restriction endonucleases that do not cut within the 16S rRNA gene of the strains. This was followed by Southern hybridization with a biotinylated probe complementary to the 16S rRNA gene. The copy number of the 16S rRNA gene within a Lactobacillus species was found to be conserved. From the hybridization results, Lactobacillus salivarius I 24 was estimated to have seven copies of the 16S rRNA gene, Lactobacillus panis C 17 to have five copies and Lactobacillus gallinarum strains I 16 and I 26 four copies. The 16S rRNA gene copy numbers of L. gallinarum and L. panis reported in the present study are the first record. Lactobacillus brevis strains I 12, I 23, I 25, I 211, I 218 and Lactobacillus reuteri strains C 1, C 10, C 16 were estimated to have at least four copies of the 16S rRNA gene. In addition, distinct rRNA restriction patterns which could discriminate the strains of L. reuteri and L. gallinarum were also detected. Information on 16S rRNA gene copy number is important for physiological, evolutionary and population studies of the bacteria.


Download File

[img]
Preview
PDF (Abstract)
Estimation of 16S RNA gene copy number in several probiotic Lactobacillus strains isolated from the gastrointestinal tract of chicken.pdf

Download (83kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
DOI Number: https://doi.org/10.1111/j.1574-6968.2008.01305.x
Publisher: Blackwell Publishing Ltd
Keywords: Lactobacillus spp.; 16S rRNA gene copy number; rRNA restriction patterns.
Depositing User: Nida Hidayati Ghazali
Date Deposited: 19 Jul 2013 08:31
Last Modified: 23 Nov 2015 07:17
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1111/j.1574-6968.2008.01305.x
URI: http://psasir.upm.edu.my/id/eprint/14568
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item