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Enzymatic synthesis of betulinic acid ester as an anticancer agent: optimization study


Citation

Ahmad @ Amat, Faujan and Moghaddam, Mansour Ghaffari and Basri, Mahiran and Abdul Rahman, Mohd Basyaruddin (2010) Enzymatic synthesis of betulinic acid ester as an anticancer agent: optimization study. Biocatalysis and Biotransformation, 28 (3). pp. 192-200. ISSN 1024-2422; ESSN: 1029-2446

Abstract

Immobilized Candida antarctica lipase, Novozym 435, was used to catalyze the esterification reaction between betulinic acid and phthalic anhydride to synthesize 3-O-phthalyl betulinic acid in n-hexane/chloroform. Response surface methodology based on a five-level, four-variable central composite rotatable design was employed to evaluate the effects of synthesis parameters such as reaction time, reaction temperature, enzyme amount and substrate molar ratio on the yield of ester. Based on the response surface model, the optimal enzymatic synthesis conditions were predicted to be: reaction time 20.3 h, reaction temperature 53.9°C, enzyme amount 145.6 mg, betulinic acid to phthalic anhydride molar ratio 1:1.11. The predicted yield was 65.8% and the actual yield was 64.7%.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Science
DOI Number: https://doi.org/10.3109/10242421003753795
Publisher: Informa Healthcare
Keywords: Response surface methodology; Central composite rotatable design; Enzymatic synthesis; Esterification; Betulinic acid
Depositing User: Najwani Amir Sariffudin
Date Deposited: 05 Mar 2014 07:06
Last Modified: 25 Apr 2016 07:03
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3109/10242421003753795
URI: http://psasir.upm.edu.my/id/eprint/14540
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