UPM Institutional Repository

Enhanced production of thermophilic xylanase by recombinant Escherichia coli DH5a through optimization of medium and dissolved oxygen level.


Citation

Basar, Basilar and Mohamed, Mohd Shamzi and Mohamed, Rosfarizan and Puspaningsih, Ni Nyoman Tri and Ariff, Arbakariya (2010) Enhanced production of thermophilic xylanase by recombinant Escherichia coli DH5a through optimization of medium and dissolved oxygen level. International Journal of Agriculture and Biology, 12 (3). pp. 321-328. ISSN 1560–8530; ESSN: 1814–9596

Abstract

Enhancement of thermophilic xylanase production by recombinant Escherichia coli DH5α through suitable medium formulation was initially investigated using shake flask cultures. Thereafter the effect of dissolved oxygen tension (DOT) level on the performance of xylanase fermentation by E. coli DH5α was investigated in 2 L stirred tank bioreactor using the optimal medium. Among the two basal medium tested (complex medium of Luria Bertani & defined mineral medium), defined mineral medium gave the highest growth and xylanase production. The optimal glucose and (NH4)2504 for xylanase production was obtained at 10 g L -1 and 2 g L-1, respectively. Growth of E. coli DH5α and xylanase production was inhibited in oxygen limited fermentation, where dissolved oxygen tension level was controlled at 0% saturation. On the other hand,xylanase production was enhanced at DOT level controlled at 20% saturation, though growth was not significantly improved. Substantially high xylanase production (1784.57 U mL -1) was obtained in fermentation using optimal medium composition and DOT level. These results indicate that efficient process control strategy is important for the mass production of xylanase enzyme by E. coli DH5α.


Download File

[img]
Preview
PDF (Abstract)
Enhanced production of thermophilic xylanase by recombinant Escherichia coli DH5a through optimization of medium and dissolved oxygen level.pdf

Download (187kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Publisher: Friends Science Publishers
Keywords: Thermophilic xylanase; Xylan-degrading enzymes; Batch fermentation; E. coli DH5a; Kinetic; Modeling.
Depositing User: Nida Hidayati Ghazali
Date Deposited: 11 Feb 2014 07:26
Last Modified: 28 Aug 2015 08:09
URI: http://psasir.upm.edu.my/id/eprint/14510
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item