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Enhanced interferon-α2b production in periplasmic space of Escherichia coli through medium optimization using response surface method


Citation

Tan, Joo Shun and Ramanan, Ramakrishnan Nagasundara and Azaman, Siti Nor Ani and Ling, Tau Chuan and Mustafa, Shuhaimi and Ariff, Arbakariya (2009) Enhanced interferon-α2b production in periplasmic space of Escherichia coli through medium optimization using response surface method. The Open Biotechnology Journal, 3. pp. 117-124. ISSN 1874-0707

Abstract

The influence of different carbon and nitrogen sources on growth of recombinant Escherichia coli and human interferon-α2b (IFN-α2b) production in periplasmic space was studied in shake flask culture. A statistical method based on Plackett-Burman design was used to screen the main medium components that greatly influenced the performance of the fermentation process. The optimization of medium was performed using response surface methodology (RSM) where three critical factors (glucose, yeast extract and peptone) were optimized using central composite design. The highest yield of periplasmic recombinant human interferon-α2b (PrIFN-α2b) (335.8 μg/L) was predicted to be obtained in optimized medium containing 5.47 g/L glucose, 55.24 g/L yeast extract and 42.27 g/L peptone. The production of IFN-α2b in periplasmic space in optimized medium was about 2.5, 11.7 and 124.4 times higher than Terrific broth (TB), Luria-Bertani (LB), and minimal medium (M9), respectively.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Engineering
Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
DOI Number: https://doi.org/10.2174/1874070700903010117
Publisher: Bentham Open
Keywords: Escherichia coli; Human interferon-α2b; Plackett-Burman; Optimization; Response surface methodology
Depositing User: Nabilah Mustapa
Date Deposited: 08 Apr 2019 08:51
Last Modified: 08 Apr 2019 08:51
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.2174/1874070700903010117
URI: http://psasir.upm.edu.my/id/eprint/14509
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