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Engineering of E. coli for increased production of L-lactic acid


Citation

Tengku Zainal Mulok, Tengku Elida and Chong, Mei Ling and Shirai, Yoshihito and Abdul Rahim, Raha and Hassan, Mohd Ali (2009) Engineering of E. coli for increased production of L-lactic acid. African Journal of Biotechnology, 8 (18). art. no. EACCE0F9284. pp. 4597-4603. ISSN 1684–5315

Abstract

An over-expressed L-ldh gene derivative of Escherichia coli BAD-ldh was developed. L-ldh gene from Enterococcus facelis KK1 consisted of an open reading frame of 954 bp encoding 316 amino acids. L-ldh gene was cloned into pBAD vector and transformed into E.coli SZ85 by electroporation. SDS-page and western blotting method confirmed the presence of recombinant L-LDH enzyme with the approximate size of 40kD. The activity of L-lactate dehydrogenase was achieved at 170 U ml¯¹. E.coli BAD85 was found to produce 0.62 g l¯¹ of lactic acid from 1 g 1¯¹ of fructose in 24 h. L-ldh gene from was successfully transformed into E.coli SZ85 with the maximum production of L-lactic acid at 0.62 g l¯¹.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Publisher: Academic Journals
Keywords: Enterobacter; Fermentation processes; Genes; Lactic acid bacteria; Molecular genetics; L-ldh gene
Depositing User: Nida Hidayati Ghazali
Date Deposited: 11 Feb 2014 03:10
Last Modified: 03 May 2016 11:13
URI: http://psasir.upm.edu.my/id/eprint/14503
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