UPM Institutional Repository

Detection of virulence genes and enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) analysis among raw vegetables isolates of Campylobacter jejuni


Citation

Abd. Mutalib, Sahilah and Tuan Soh, Tuan Suraya and Ismail, Noraida and Ariffin, Ahmad Azuhairi and Chai, Lay Ching and Radu, Son (2010) Detection of virulence genes and enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) analysis among raw vegetables isolates of Campylobacter jejuni. International Food Research Journal, 17 (3). pp. 681-690. ISSN 1985-4668; ESSN: 2231-7546

Abstract

A total of 20 (n=20) raw vegetable isolates of Campylobacter jejuni were examined for their virulence genes and genetic diversity by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) analysis. All raw vegetable isolates of C. jejuni were encoded cadF and ceuE genes. None of the C. jejuni isolates was cdtA positive. Whereas, 12 (12/20) isolates were positive for cdtB and 6 (6/20) were positive for cdtC. All the 20 isolates of C. jejuni were subtyped and they produced 18 ERIC-PCR profiles namely E1 to E18. Dendrogram performed from cluster analysis showed that the 20 isolates were grouped into 2 major clusters, cluster I and II indicating the high level of local geographical genetic variation. The detection of virulence genes among C. jejuni strains isolated from raw vegetables raised concern on the threat of campylobacteriosis.


Download File

Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Faculty of Medicine and Health Science
Publisher: Faculty of Food Science and Technology, Universiti Putra Malaysia
Keywords: Virulence genes; ERIC-PCR; Raw vegetables; Campylobacter jejuni
Depositing User: Raja Norazlinda Raja Azenam
Date Deposited: 30 Apr 2014 09:15
Last Modified: 25 Sep 2015 06:59
URI: http://psasir.upm.edu.my/id/eprint/13940
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item