Citation
Abstract
Subtype-specific multiplex reverse transcription-polymerase chain reaction (RT-PCR) was developed to simultaneously detect three subtypes (H5, H7 and H9) of avian influenza virus (AIV) type A. The sensitivity of the multiplex RT-PCR was evaluated and compared to that of RT-PCR–enzyme-linked immunosorbent assay (ELISA) and conventional RT-PCR. While the sensitivity of the multiplex RT-PCR is as sensitive as the conventional RT-PCR, it is 10 times less sensitive than RT-PCR–ELISA. The multiplex RT-PCR is also as sensitive as the virus isolation method in detecting H9N2 from tracheal samples collected at day 3 and 5 post inoculation. Hence, the developed multiplex RT-PCR assay is a rapid, sensitive and specific assay for detecting of AIV subtypes.
Download File
|
Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Veterinary Medicine Faculty of Biotechnology and Biomolecular Sciences Institute of Bioscience |
| DOI Number: | https://doi.org/10.1016/j.micres.2007.01.001 |
| Publisher: | Elsevier |
| Keywords: | H9N2 subtype; Multiplex RT-PCR; Type A avian influenza virus infections. |
| Depositing User: | Azizan Arshad |
| Date Deposited: | 09 Dec 2013 03:51 |
| Last Modified: | 02 Sep 2015 03:32 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.micres.2007.01.001 |
| URI: | http://psasir.upm.edu.my/id/eprint/13933 |
| Statistic Details: | View Download Statistic |
Actions (login required)
 |
View Item |