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Detection of diarrheagenic Escherichia coli isolated using molecular approaches.


Abdullah Al-Haj, Nagi Ahmed and Shamsudin, Mariana Nor and Abdul Rahim, Raha and Ishak, Zamri (2008) Detection of diarrheagenic Escherichia coli isolated using molecular approaches. Research Journal of Microbiology, 3 (5). pp. 359-367. ISSN 1816-4935


Escherichia coli strains are among the major bacterial causes of diarrheal illness. There are now seven classes of diarrheagenic E. coli (DEC), namely enteropathogenic E. coli (EPEC), enterohaemorrhagic E. coli (EHEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC), enteroaggregative E. coli (EAEC), diarrhea-associated hemolytic E. coli (DHEC) and Cytolethal Distending Toxin (CDT)-producing E. coli. Due to the need for costly and labor-intensive diagnostic procedures, identification of DEC is difficult at standard laboratories. Therefore, Polymerase Chain Reaction (PCR) or dot blot has been used for genetic detection of DEC of 25 E. coli isolates from different sources. Amplification of eae (277 bp), bfp (266 bp), stx1 (154 bp), EAST (94 bp), stx2 (698 bp) and elt (450 bp) genes of a single product in separate reactions was produced. PCR showed ability to amplify and detected genes of the most common important categories of diarrheagenic E. coli isolates of different sources, it is possible implementation of this technique to diagnosis water, foodborne outbreaks related to E. coli. Dots blot and sequence analysis used to confirm the results of PCR.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.3923/jm.2008.359.367
Publisher: Academic Journals
Keywords: Cytolethal distending toxins; Diarrheagenic E. coli; Diarrheal illness; Enteroinvasive E. coli; Escherichia coli.
Depositing User: Raja Norazlinda Raja Azenam
Date Deposited: 29 Apr 2014 11:58
Last Modified: 02 Dec 2015 09:00
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3923/jm.2008.359.367
URI: http://psasir.upm.edu.my/id/eprint/13931
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