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Defoliation of in vitro shootlets of Azadirachta excelsa (Jack) M. Jacobs: a possible solution


Ab. Shukor, Nor Aini and Jainol, Judy E. and Mohd Yusof, Aziah and Abdul Kadir, Mihdzar (2008) Defoliation of in vitro shootlets of Azadirachta excelsa (Jack) M. Jacobs: a possible solution. The Malaysian Forester, 71 (1). pp. 37-44. ISSN 0302-2935


Defoliation is a common phenomenon which usually hinders the productivity of plantlets of Azadirachta excelsa through micropropagation. Thus, a study to develop a procedure to overcome defoliation of in vitro shootlets of A. excelsa after undergoing rooting stage was conducted. Shoots exhibiting defoliation were transferred on to MS medium supplemented either with only 0.24% gelrite, mixture of 0.1 mgl-1 BAP, 1.0 mgl-1 NAA and 0.75% difco-bacto agar, mixture of 0.24% gelrite and 0.2% activated charcoal and mixture of 0.24% gelrite, 2.0 mgl-1 BAP and 10.0 mgl-1 AgNO3. The results indicated that the different treatment produced significant effects at P<0.05 on the number of leaves, shoot length, root length and number of axillary shoots after 30 days of culture incubation. The MS medium supplemented only with 0.24% gelrite was the most effective treatment producing the highest number of leaves (9.8) and the longest shoot and root lengths of 17.2 mm and 43.5 mm respectively. The addition of NAA, BAP and AgNO3 stimulated callus formation and growth of axillary shoot.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Forestry
Publisher: Forestry Department Peninsular Malaysia
Keywords: Azadirachta excelsa; Micropropagation; Defoliation; In vitro shootlets; Gelrite
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 18 Jun 2015 01:47
Last Modified: 02 Dec 2015 07:07
URI: http://psasir.upm.edu.my/id/eprint/13882
Statistic Details: View Download Statistic

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