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Cytotoxicity, cell cycle arrest, and apoptosis in breast cancer cell lines exposed to an extract of the seed kernel of Mangifera pajang (bambangan).


Citation

Abu Bakar, Mohd Fadzelly and Mohamad, Mariati and Rahmat, Asmah and Burr, Steven A. and Fry, Jeffrey R. (2010) Cytotoxicity, cell cycle arrest, and apoptosis in breast cancer cell lines exposed to an extract of the seed kernel of Mangifera pajang (bambangan). Food and Chemical Toxicology, 48 (6). pp. 1688-1679. ISSN 0278-6915; ESSN: 1873-6351

Abstract

An extract of Mangifera pajang kernel has been previously found to contain a high content of antioxidant phytochemicals. The present research was conducted to investigate the anticancer potential of this kernel extract. The results showed that the kernel crude extract induced cytotoxicity in MCF-7 (hormone-dependent breast cancer) cells and MDA-MB-231 (non-hormone dependent breast cancer) cells with IC50 values of 23 and 30.5μg/ml, respectively. The kernel extract induced cell cycle arrest in MCF-7 cells at the sub-G1 (apoptosis) phase of the cell cycle in a time-dependent manner. For MDA-MB-231 cells, the kernel extract induced strong G2-M arrest in cell cycle progression at 24. h, resulting in substantial sub-G1 (apoptosis) arrest after 48 and 72. h of incubation. Staining with Annexin V-FITC and propidium iodide revealed that this apoptosis occurred early in both cell types, 36. h for MCF-7 cells and 24. h for MDA-MB-231cells, with 14.0% and 16.5% of the cells respectively undergoing apoptosis at these times. This apoptosis appeared to be dependent on caspase-2 and -3 in MCF-7 cells, and on caspase-2, -3 and -9 in MDA-MB-231 cells. These findings suggest that M. pajang kernel extract has potential as a potent cytotoxic agent against breast cancer cell lines.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1016/j.fct.2010.03.046
Publisher: Elsevier
Keywords: Apoptosis; Breast cancer; Cytotoxicity; Mangifera pajang.
Depositing User: Raja Norazlinda Raja Azenam
Date Deposited: 29 Apr 2014 11:17
Last Modified: 23 Oct 2015 03:18
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.fct.2010.03.046
URI: http://psasir.upm.edu.my/id/eprint/13865
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