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Cytotoxic activity of coumarins from Micromelium minutum


Susidarti, Ratna Asmah and Rahmani, Mawardi and Mohd Ismail, Hazar Bebe and Md. Sukari, Mohd Aspollah and Yap, Taufiq Yun Hin and Ee, Gwendoline Cheng Lian and Ali, Abdul Manaf (2009) Cytotoxic activity of coumarins from Micromelium minutum. Pharmaceutical Biology, 47 (2). pp. 182-185. ISSN 1388-0209; ESSN: 1744-5116


The crude petroleum ether and chloroform extracts of Micromelum minutum (G. Frost.) Wright & Arn (Rutaceae) showed strong cytotoxic activity when tested against a T-lymphoblastic leukemia cell line. Further fractionation of the extracts resulted in the isolation of five new coumarins 3″,4″-dihydrocapnolactone, 2′,3′-epoxyisocapnolactone, 8-hydroxyisocapnolactone-29,39-diol, 8-hydroxy-3″,4″-dihydrocapnolactone-29,39-diol and 8,4″-dihydroxy-3″,4″-dihydrocapnolactone-29,39-diol, and two triterpenes. Some of these compounds were strongly active against T-lymphoblastic leukemia (CEM-SS), promyeolocytic leukemia (HL60), cervical cancer (HeLa) and liver cancer (HepG2) cell lines. 8-Hydroxyisocapnolactone-29,39-diol was found to be the most active with IC50 values of 2.9, 2.5, 6.9, and 5.9 μg/ml, respectively. This was followed by 2′,3′-epoxyisocapnolactone. When evaluated against the normal mouse fibroblast (3T3) cell line, 8-hydroxyisocapnolactone-29,39-diol was found to be inactive, hence it could serve as a valuable lead for further design and synthesis of more active analogues.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Science
DOI Number: https://doi.org/10.1080/13880200802436513
Publisher: Informa Healthcare
Keywords: Capnolactone; Coumarins; Cytotoxicity; 2′,3′-epoxyisocapnolactone; 8-hydroxyisocapnolactone 29; 39-diol; Micromelum minutum; Triterpenes
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 18 Jun 2015 01:06
Last Modified: 29 Nov 2016 09:10
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/13880200802436513
URI: http://psasir.upm.edu.my/id/eprint/13854
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