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CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression.


Citation

Hyde, Stephen C. and Pringle, Ian A. and Abdullah, Syahril and Lawton, Anna E. and Davies, Lee A. and Varathalingam, Anusha and Nunez-Alonso, Graciela A. and Green, Anne Marie and Bazzani, Reto P. and Sumner-Jones, Stephanie G. and Chan, Mario and Hong, Yu Li and Yew, Nelson S. and Cheng, Seng H. and Boyd, Christopher A. and Davies, Jane C. and Griesenbach, Uta and Porteous, David John and Sheppard, David N. and Munkonge, Felix M. and Alton, Eric W. F. W. and Gill, Deborah R. (2008) CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. Nature Biotechnology, 26 (5). pp. 549-551. ISSN 1087-0156; ESSN: 1546-1696

Abstract

Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammatory response, whereas CpG-free pDNA vectors do not. Using a CpG-free pDNA expression vector, we achieved sustained (≥56 d) in vivo transgene expression in the absence of lung inflammation.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1038/nbt1399
Publisher: Nature Publishing Group
Keywords: CpG; Gene therapy; Inflammation; Lung; Plasmids.
Depositing User: Raja Norazlinda Raja Azenam
Date Deposited: 09 Sep 2014 07:38
Last Modified: 17 Sep 2015 00:37
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1038/nbt1399
URI: http://psasir.upm.edu.my/id/eprint/13817
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