UPM Institutional Repository

Controlled expression of cholera toxin B subunit from Vibro cholerae in Escherichia coli.


Citation

Toosa, Haryanti and Shamsudin , Mariana Nor and Saiful Yazan, Latifah and Yusoff, Khatijah and Abdul Rahim, Raha (2008) Controlled expression of cholera toxin B subunit from Vibro cholerae in Escherichia coli. Pakistan Journal of Biological Sciences, 11 (13). pp. 1718-1722. ISSN 1028-8880; ESSN: 1812-5735

Abstract

The ctxB gene, the causative agent of cholera epidemic was successfully cloned from V. cholerae in E. coli. The insertion of the gene was confirmed by PCR as well as restriction digestion analyses. The sequencing results for the gene confirmed that the insert was in the correct orientation and in-frame with the P(BAD) promoter and it showed that the gene was 99% homologous to the published ctxB sequence. The CTB protein was successfully expressed in E. coli using the pBAD/His vector system. The expected protein of approximately 14 kDa was detected by SDS-PAGE and Western blot. The use of pBAD/His vector to express the cholera toxin gene in E. coli would facilitate future study of toxin gene products.


Download File

[img]
Preview
PDF (Abstract)
Controlled expression of cholera toxin B subunit from Vibro cholerae in Escherichia coli.pdf

Download (83kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Medicine and Health Science
Institute of Bioscience
Publisher: Asian Network for Scientific Information
Keywords: Ctx; Expression; Escherichia coli; Cholera.
Depositing User: Nida Hidayati Ghazali
Date Deposited: 12 Feb 2014 04:02
Last Modified: 20 Jan 2016 03:50
URI: http://psasir.upm.edu.my/id/eprint/13785
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item