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Comparison on the effect of treatment and subculturing on shoot regeneration from shoot tip seedlings of Psidium guajava L. var. Beaumont


Citation

Abdullah, Tajul Afif and Abdul Aziz, Maheran and Abdul Rashid, Azmi and Saleh, Ghizan and Elhory, Suleiman Mohamed Ahmed and Panjaitan, Syaiful Bahri and Sajili, Mohammad Hailmi and Mohamad, Noor Muzamil (2009) Comparison on the effect of treatment and subculturing on shoot regeneration from shoot tip seedlings of Psidium guajava L. var. Beaumont. Pakistan Journal of Biotechnology, 6 (1-2). pp. 21-26. ISSN 1812-1837; ESSN: 2312-7791

Abstract

An experiment was carried out in order to study the effect of treatment containing 0.5 and 1.0 mg/L 6-benzylaminopurine (BAP) and effect of subculture on percentage of shoot formation, number of shoot per explant and mean shoot height of shoot tip explant of Psidium guajava L. var. Beaumont. MS medium (Murashige and Skoog, 1962) was used with 30 g/L of sucrose and 7 g/L agar type 900. Shoot tips of elongated shoots were decapitated to abolish its apical dominance prior to sub-culturing was carried out to a fresh medium every 4 weeks. The increase in mean number of shoots per explant and the shoot height was observed for every subculture. Treatment 1.0 mg/L BAP in comparison to treatment 0.5 mg/L BAP showed higher mean number of shoot per explant per subculture whereas treatment 0.5 mg/L BAP showed higher mean shoot height per subculture. Data were analyzed using T-test until the fifth subculture.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Publisher: Institute of Biotechnology and Genetic Engineering
Keywords: Treatment; Subculturing; Shoot regeneration; Shoot tip seedling; Psidium guajava L. var. Beaumont
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 18 Jan 2016 08:33
Last Modified: 18 Jan 2016 08:33
URI: http://psasir.upm.edu.my/id/eprint/13713
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