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Comparison of expression systems for the production of human interferon-a2b.


Memari, Hamid Rajabi and Ramanan, Ramakrishnan Nagasundara and Ariff, Arbakariya (2010) Comparison of expression systems for the production of human interferon-a2b. Central European Journal of Biology, 5 (4). pp. 446-455. ISSN 1895-104X


The production of human interferon alpha2b (IFN-α2b) in two expression systems, tobacco (Nicotiana tabaccum) and Escherichia coli, was compared in various aspects such as safety, yield, quality of product and productivity. In the E. coli system, IFN-α2b was expressed under a pelB signal sequence and a T7lac promoter in a pET 26b(+) vector. The same gene was also cloned in expression plant vector (pCAMBIA1304) between cauliflower mosaic virus promoter (CaMV35S) and poly A termination region (Nos) and expressed in transgenic tobacco plants. The expression of protein in both systems was confirmed by western immunoblotting and the quantity of the protein was determined by immunoassay. The amount of periplasmic expression in E. coli was 60 µg/L of culture, while the amount of nuclear expression in the plant was 4.46 µg/kg of fresh leaves. The result of this study demonstrated that IFN-α2b was successfully expressed in periplasm of bacterial and plant systems. The limitations on the production of IFN-α2b by both systems are addressed and discussed to form the basis for the selection of the appropriate expression platform.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
DOI Number: https://doi.org/10.2478/s11535-010-0036-y
Publisher: SP Versita
Keywords: Expression platform; Interferon-a2b; Scherchia coli; Transgenic tobacco.
Depositing User: Ms. Nida Hidayati Ghazali
Date Deposited: 23 Jul 2013 03:52
Last Modified: 21 Oct 2015 00:09
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.2478/s11535-010-0036-y
URI: http://psasir.upm.edu.my/id/eprint/13689
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