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Comparative evaluation of different cell disruption methods for the release of recombinant hepatitis B core antigen from Escherichia coli


Citation

Ho, Chin Woi and Tan, Wen Siang and Yap, Wei Boon and Ling, Tau Chuan and Tey, Beng Ti (2008) Comparative evaluation of different cell disruption methods for the release of recombinant hepatitis B core antigen from Escherichia coli. Biotechnology and Bioprocess Engineering, 13 (5). pp. 577-583. ISSN 1226-8372; ESSN: 1976-3816

Abstract

A comparative evaluation of five different cell-disruption methods for the release of recombinant hepatitis B core antigen (HBcAg) from Escherichia coli was investigated. The cell disruption techniques evaluated in this study were high-pressure homogenization, batch-mode bead milling, continuous-recycling bead milling, ultrasonication, and enzymatic lysis. Continuous-recycling bead milling was found to be the most effective method in terms of operating cost and time. However, the highest degree of cell disruption and amounts of HBcAg were obtained from the high-pressure homogenization process. The direct purification of HBcAg from the unclarified cell disruptate derived from high-pressure homogenization and bead milling techniques, using batch anion-exchange adsorption methods, showed that the conditions of cell disruption have a substantial effect on subsequent protein recovery steps.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Engineering
Institute of Bioscience
DOI Number: https://doi.org/10.1007/s12257-008-0020-9
Publisher: Korean Society for Biotechnology and Bioengineering
Keywords: Batchanion-exchange adsorption; Bead milling; Hepatitis B core antigen; High-pressure homogenization; Lysozyme; Ultrasonication
Depositing User: Nabilah Mustapa
Date Deposited: 15 May 2015 12:21
Last Modified: 17 Sep 2015 00:24
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s12257-008-0020-9
URI: http://psasir.upm.edu.my/id/eprint/13664
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