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Characterization of a diesel-degrading strain isolated from a hydrocarbon-contaminated site


Citation

Abd. Shukor, Mohd. Yunus and Dahalan, Farrah Aini and Jusoh, Arif Zaidi and Muse, Radzali and Shamaan, Nor Aripin and Syed, Mohd Arif (2009) Characterization of a diesel-degrading strain isolated from a hydrocarbon-contaminated site. Journal of Environmental Biology, 30 (1). pp. 145-150. ISSN 0254-8704; ESSN: 2394-0379

Abstract

A diesel-degrading bacterium has been isolated from a diesel-polluted site. The isolate was tentatively identified as Staphylococcus aureus strain DRY11 based on partial 16S rDNA molecular phylogeny and Biolog® GP microplate panels and Microlog® database. Isolate 11 showed an almost linear increase in cellular growth with respect to diesel concentrations with optimum growth occurring at 4% (v/v) diesel concentration. Optimization studies using different nitrogen sources showed that the best nitrogen source was potassium nitrite. Sodium nitrite was optimum at 1.2 g l-1 and higher concentrations were strongly inhibitory to cellular growth. The optimal pH that supported growth of the bacterium was between 7.5 to 8.0 and the isolate exhibited optimal broad temperature supporting growth on diesel from 27 to 37 °C. An almost complete removal of diesel components was seen from the reduction in hydrocarbon peaks observed using Solid Phase Microextraction Gas Chromatography analysis after 5 days of incubation. The characteristics of this bacterium suggest that it is suitable for bioremediation of diesel spills and pollutions in the tropics.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Publisher: Triveni Enterprises
Keywords: Isolation; Characterization; Diesel-degrading; S. aureus
Depositing User: Nida Hidayati Ghazali
Date Deposited: 22 Jul 2013 03:55
Last Modified: 14 Jul 2020 03:10
URI: http://psasir.upm.edu.my/id/eprint/13535
Statistic Details: View Download Statistic

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