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Biological and phytochemical investigations of Goniothalamus umbrosus leaves hexane extract.


Citation

Abdelwahab, Siddig Ibrahim and Abdul, Ahmad Bustamam and Elhassan Taha, Manal Mohamed and Mohan, Syam Murali and Al-Zubairi, Adel Sharaf and Abdullah Al-Haj, Nagi Ahmed and Abdullah, Rasedee and Adam Mariod, Abdelbasit (2009) Biological and phytochemical investigations of Goniothalamus umbrosus leaves hexane extract. Journal of Medicinal Plants Research, 3 (11). pp. 880-885. ISSN 1996-0875

Abstract

Antibacterial, antioxidant, anticancer properties and chemical compositions of Goniothalamus umbrosus (GU) hexane extract was investigated using disc diffusion method, DPPH assay, MTT cytotoxicity test (MCF-7 breast cancer cell line, HT-29 colon cancer cell line and CEMss leukemia cell line) and GC-MS, respectively. Anti-tumor effect of GU was further confirmed morphologically under inverted and fluorescent microscopy. Anticancer properties were only observed on MCF-7 with an IC50 of 20 ± 4.469 μg/ml. Morphology of MCF-7, after exposure to the extract, has suggested strongly the incidence of a cell death that might resemble to apoptosis. Antioxidant activity was not comparable significantly to the commercial standard antioxidant butylated hydroxytoluene (BHT). The extract failed to exhibit any antibacterial activity towards two Gram-positive bacteria, Methicillin Resistant Staphylococcus aureus (MRSA) and Bacillus subtilis B29, and other two Gram-negative bacteria, Pseudomonas aeruginosa 60690 and Salmonella choleraesuis. Analyses of the extract by gas chromatography and GC-mass spectrometry (GC-MS) tentatively identified 68 compounds, including a group naphthalene derivatives (18.33%) and eudesma-4(14),7(11)-diene (5.97%). A further research is recommended to verify the mechanism of oncolytic action of the hexane extract of G. umbrosus


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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Publisher: Academic Journals
Keywords: Anti-oxidant; anti-tumor; Antibacterial; GC-MS; Goniothalamus umbrosus.
Depositing User: Azizan Arshad
Date Deposited: 09 Dec 2013 05:13
Last Modified: 19 Nov 2015 09:06
URI: http://psasir.upm.edu.my/id/eprint/13418
Statistic Details: View Download Statistic

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