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Association of Helicobacter pylori with colorectal cancer development.


Fouad Alsamaka, Fadi and Abdulamir, Ahmed Sahib and Khalid Mahdi, Laila and Alnaib, Khalid and Abu Bakar, Fatimah (2010) Association of Helicobacter pylori with colorectal cancer development. Asian Biomedicine, 4 (4). pp. 609-618. ISSN 1905-7415; ESSN:1875-855X


Background: Helicobacter pylori (H. pylori) may be associated with colorectal cancer. However, the underlying mechanisms are still unclear. Objectives: Explore the serostatus of H. pylori cytotoxicity-associated gene A product (CagA) in patients with colorectal carcinoma, and assess the association of H. pylori with colorectal cancer via c-Myc and MUC-2 proteins at tumor tissues. Methods: H. pylori CagA IgG antibodies were screened using enzyme-linked immunosorbent assay (ELISA) in 30 patients with colorectal carcinoma and 30 cancer-free control subjects. Paraffin-embedded blocks were examined for the expression of c-Myc and MUC-2 protein by immunohistochemistry. Results: H. pylori CagA seropositivity increased significantly among colorectal cancer patients (p <0.05). The expression of c-Myc and MUC-2 in colorectal carcinoma patients was over-expressed (80%), and downexpressed (63%) in resection margins (p <0.05). c-Myc over-expression and MUC-2 down-expression were associated with CagA-positive rather than CagA-negative H. pylori patients. In 16 CagA seropositive vs. 14 CagA seronegative patients, the expression rate was 97.3% vs. 64.2% and 33.3% vs. 78.5% for cMyc and MUC-2, respectively. CagA IgG level was significantly higher in positive than in negative c-Myc patients (p= 0.036), and in negative than in positive MUC-2 patients (p= 0.044). c-Myc and MUC-2 were positively and inversely correlated with CagA IgG level (p <0.05). Conclusions: CagA-seropositive H. pylori is most probably associated with colorectal cancer development. Part of the underlying mechanism for such association might be via alterations in expression of MUC-2, which depletes the mucous protective layer in the colo-rectum, and c-Myc, which stimulates the growth of cancerous cells.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Publisher: IOS Press
Keywords: Helicobacter pylori; Colorectal cancer; Cag A; Muc-2.
Depositing User: Khairil Ridzuan Khahirullah
Date Deposited: 10 Dec 2013 03:18
Last Modified: 22 Sep 2015 03:42
URI: http://psasir.upm.edu.my/id/eprint/13314
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